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In Vivo IS6110 Profile Changes in a Mycobacterium tuberculosis Strain as Determined by Tracking over 14 Years

María Isabel Millán-Lou1, Isabel Otal2, María Luisa Monforte3

  • 1Instituto de Investigación Sanitaria Aragón, Zaragoza, Spain Instituto Aragonés de Ciencias de la Salud, Zaragoza, Spain Hospital Universitario Miguel Servet, Zaragoza, Spain CIBER de Enfermedades Respiratorias, Zaragoza, Spain.

Journal of Clinical Microbiology
|May 8, 2015
PubMed

Insights

Transposition and homologous recombination of IS6110 insertion sequences were observed in Mycobacterium tuberculosis during sequential infections. A specific variant showed IS6110 integration within the origin of replication (oriC) region.

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • IS6110 is a major cause of genomic instability in Mycobacterium tuberculosis.
  • In vivo sequential infections provide a model to study dynamic genetic events in M. tuberculosis.

Purpose of the Study:

  • To investigate the transposition and homologous recombination of IS6110 during in vivo sequential infections of M. tuberculosis.
  • To analyze a specific M. tuberculosis Zaragoza strain variant with IS6110 integrated into the oriC region.

Main Methods:

  • Analysis of IS6110 activity in different clones of M. tuberculosis Zaragoza.
  • Focus on genetic characterization of a variant with IS6110 integration at the oriC locus.

Main Results:

  • Transposition and homologous recombination of IS6110 were detected during sequential in vivo infections.
  • A specific variant exhibited IS6110 integration within the origin of replication (oriC) region.

Conclusions:

  • IS6110 exhibits dynamic behavior, including transposition and recombination, during M. tuberculosis infection.
  • Integration of IS6110 into the oriC region represents a significant genetic alteration with potential functional implications.

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