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Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria
Published on: August 13, 2021
Jiangella mangrovi sp. nov., isolated from mangrove soil
Paweena Suksaard1, Kannika Duangmal2,1, Rattanaporn Srivibool3
1Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Abstract:
An aerobic, Gram-stain-positive actinomycete, designated strain 3SM4-07T, was characterized using a polyphasic taxonomic approach. The strain produced branching mycelium which fragmented into short or elongated rods. The whole-cell hydrolysates contained ll-2,6-diaminopimelic acid as the diagnostic diamino acid, with glucose and ribose as the main sugars. The predominant cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0.The predominant menaquinone was MK-9(H4). Phospholipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and phosphatidylinositol mannoside. Mycolic acids were absent. The DNA G+C content was 72.3 mol%. Strain 3SM4-07T formed a phylogenetic line within the genus Jiangella and its 16S rRNA gene sequence was related most closely to Jiangella alkaliphila D8-87T (99.0% similarity), Jiangella muralis 15-Je-017T (98.8%), Jiangella alba YIM 61503T (98.6%) and Jiangella gansuensis YIM 002T (98.6%). However, mean DNA-DNA hybridization values revealed that strain 3SM4-07T differed from the closest species previously described in this genus. Data from phenotypic, chemotaxonomic and molecular analyses between strain 3SM4-07T and recognized species of the genus Jiangella indicate that strain 3SM4-07T is a representative of a novel species of the genus Jiangella, for which the name Jiangella mangrovi sp. nov. is proposed. The type strain is 3SM4-07T ( = BCC 60398T = NBRC 109648T).
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