Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conserved Binding Sites01:49

Conserved Binding Sites

5.3K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: INO80 regulates promoter-associated R-loops to coordinate transcription and maintain genome stability in embryonic stem cells.

Biological research·2026
Same author

Does body composition influence air force pilots' resistance to high-G acceleration? A systematic review meta-analysis.

iScience·2026
Same author

Association of revised morphological uterus sonographic assessment (MUSA) features of adenomyosis and IVF outcomes.

Scientific reports·2026
Same author

Estrogen Regulates Microtubule-Associated Protein 1 Light Chain 3 in Mouse Uterine Endometrial Epithelium.

Development & reproduction·2026
Same author

Exosome-Mediated miRNA Delivery Restores Early Differentiation and Survival Programs in DGCR8-Deficient Mouse Embryonic Stem Cells.

International journal of molecular sciences·2026
Same author

Expression of purinergic receptor P2Y, G-protein coupled 2, is regulated by estrogen during the estrous cycle in mouse uterus.

Purinergic signalling·2026

Related Experiment Video

Updated: Apr 12, 2026

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.9K

Identification and Characterization of LHX8 DNA Binding Elements.

Miree Park1, Sanghyun Jeon1, Ji-Hye Jeong1

  • 1Dept. of Biomedical Science, CHA University, Seoul 135-081, Korea.

Development & Reproduction
|May 8, 2015
PubMed
Summary

Researchers identified the specific DNA sequence (TGATTG) that the LIM homeobox 8 (Lhx8) gene binds to. This discovery clarifies how Lhx8 regulates gene transcription in oocytes during early follicle development.

Keywords:
FolliculogenesisLhx8Transcription factor

More Related Videos

A Rapid In Vivo Bioassay for Developmentally Active Enhancers
00:08

A Rapid In Vivo Bioassay for Developmentally Active Enhancers

1.5K
An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
09:58

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

Published on: November 29, 2016

17.1K

Related Experiment Videos

Last Updated: Apr 12, 2026

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.9K
A Rapid In Vivo Bioassay for Developmentally Active Enhancers
00:08

A Rapid In Vivo Bioassay for Developmentally Active Enhancers

1.5K
An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
09:58

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

Published on: November 29, 2016

17.1K

Area of Science:

  • Reproductive Biology
  • Molecular Genetics
  • Developmental Biology

Background:

  • The LIM homeobox 8 (Lhx8) gene is crucial for mammalian folliculogenesis, with preferential expression in germ cells.
  • The specific DNA sequences recognized and bound by Lhx8 have not been previously characterized.
  • Understanding Lhx8's DNA binding is essential for elucidating its role in germ cell development.

Purpose of the Study:

  • To identify and characterize the cis-acting DNA sequence recognized by the germ-cell specific transcription factor, Lhx8.
  • To confirm the binding affinity and transcriptional activity of Lhx8 to the identified DNA element.

Main Methods:

  • Cyclic Amplification of Sequence Target (CAST) Analysis was employed to identify potential Lhx8 DNA binding elements.
  • Electrophoretic Mobility Shift Assay (EMSA) was utilized to assess the specificity of Lhx8 binding.
  • Luciferase assays were performed to evaluate the transcriptional activity mediated by Lhx8 through the identified binding site.

Main Results:

  • A specific cis-acting DNA sequence, TGATTG, was identified as the Lhx8 DNA binding element (LBE).
  • Lhx8 demonstrated high-affinity binding to the identified LBE.
  • The presence of the LBE significantly augmented the transcriptional activity of a luciferase reporter gene.

Conclusions:

  • Lhx8 directly binds to the TGATTG sequence (LBE) with high affinity.
  • Lhx8 binding to the LBE enhances gene transcription.
  • These findings establish that Lhx8 directly regulates genes containing the LBE in oocytes, playing a key role in early folliculogenesis.