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Updated: Apr 12, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
An ionically tagged water-soluble artificial enzyme promotes the dephosphorylation reaction with nitroimidazole:
José G L Ferreira1, Luciana M Ramos2, Aline L de Oliveira2
1†Department of Chemistry, Universidade Federal do Paraná (UFPR), CP 19081, CEP 81531-990, Curitiba, PR, Brazil.
Abstract:
In this paper, we describe a novel synthesized ionically tagged water-soluble artificial enzyme (PI) that can efficiently cleave phosphate esters, with enhanced an ionic liquid effect through cooperative effects for the substrate activation and further nucleophilic reaction. The dephosphorylation reaction with PI was evaluated in the presence and absence of 2-methyl-4(5)-nitroimidazole, showing impressive rate enhancements of up to 2 × 10(6)-fold, ascribed to the imidazolide species known as excellent nucleophiles, and formed favorably at lower pH values in the presence of PI.
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