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One-step purification by high-performance liquid chromatography of retinal S-antigen
C Molinotti1, P Puddu, S Perugini
1Istituto di Clinica Oculistica, Università degli Studi di Bologna.
La Ricerca in Clinica E in Laboratorio
|July 1, 1989
Abstract:
A number of subsequent chromatographic techniques are commonly used, over a period of a few days, in order to obtain pure retinal S-antigen (S-Ag), a 50,000-dalton molecular weight protein with strong uveitopathogenic properties. A rapid high-performance liquid chromatographic technique employing a TSK-G3000 SWG gel-filtration column is described for the purification of bovine S-Ag. This single chromatographic step produced S-Ag preparations of high purity and recovery when compared to the conventionally isolated S-Ag.