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Updated: Apr 12, 2026

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
Published on: March 2, 2020
Mechanisms involved in quinolone resistance in Mycoplasma mycoides subsp. capri
Nuno T Antunes1, Patrícia Assunção1, José B Poveda1
1Unidad de Epidemiología y Medicina Preventiva, Instituto Universitario de Sanidad Animal (IUSA), Universidad de Las Palmas de Gran Canaria, 35413 Arucas, Spain.
Abstract:
Mycoplasma mycoides subsp. capri is a causative agent of contagious agalactia in goats. In this study, M. mycoides subsp. capri mutants were selected for resistance to fluoroquinolones (norfloxacin, enrofloxacin and ciprofloxacin) by serial passes in broth with increasing concentrations of antibiotic. Mutations conferring cross-resistance to the three fluoroquinolones were found in the quinolone resistance determining regions of the four genes encoding DNA gyrase and topoisomerase IV. Different mutations in the DNA gyrase GyrA subunit suggest a different mechanism of inhibition between norfloxacin and the other tested fluoroquinolones. The presence of an adenosine triphosphate-dependent efflux system was suggested through the use of the inhibitor orthovanadate.
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