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Determination of homocysteine in plasma by ion-exchange chromatography
A Andersson1, L Brattström, A Isaksson
1Department of Clinical Chemistry, University Hospital, Lund, Sweden.
Scandinavian Journal of Clinical and Laboratory Investigation
|September 1, 1989
Abstract:
A rapid ion-exchange chromatographic method for determination of total homocysteine in plasma is presented. The method has a coefficient of variation of approximately 5%. A comparison with a slower method previously used in our laboratory showed a high correlation (r = 0.993). The risk of oxidation of homocysteine during the chromatography and the necessary level of dithiotreithol to prevent oxidation are also established.