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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

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Confirmation of high specificity of an automated enzyme immunoassay test for serological diagnosis of syphilis: retrospective evaluation versus results after implementation.

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Successful treatment of neuroborreliosis in an HIV patient with simultaneous Borrelia, HIV-1 and Epstein-Barr virus genomes in liquor.

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Related Experiment Video

Updated: Apr 12, 2026

Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research

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Ad36: skip serology, NAT we need

Valère J Goossens¹

¹Windmolenstraat 17, B-3690 Zutendaal, Belgium.

Journal of Diabetes and Its Complications

|May 9, 2015

Summary

No abstract available in PubMed .

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