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Updated: Apr 12, 2026

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

Published on: January 10, 2025

A hollow-fibre column system to effectively prepare washed platelets.

S Tanaka¹, T Hayashi¹, S Sugaya²

¹Japanese Red Cross Kinki Block Blood Centre, Ibaraki, Osaka, Japan.

|May 9, 2015

Summary

A new hollow-fibre column system effectively prepares washed platelet concentrates (WPCs), removing over 98% of plasma while maintaining high platelet recovery and function during storage.

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Area of Science:

Biomedical Engineering
Hematology
Transfusion Medicine

Background:

Developing effective methods for preparing washed platelet concentrates (WPCs) is crucial for transfusion safety.
Existing methods for washing platelets can be labor-intensive or may impact platelet viability.

Purpose of the Study:

To evaluate the efficacy of a novel hollow-fibre column system for preparing WPCs.
To assess the quality and in vitro characteristics of platelets processed by this system.

Main Methods:

Quantified platelet (PLT) recovery and residual plasma proteins pre- and post-wash.
Assessed in vitro PLT characteristics, including CD62P expression, aggregability, hypotonic shock response, and swirling scores, after 5 days of storage.

Main Results:

Keywords:

hollow fibre platelet transfusion reactions washed platelets

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The hollow-fibre system removed >98% of plasma with an average PLT recovery of 97%.
Washed PLTs showed comparable or improved characteristics compared to pre-washed and manually washed platelets, maintaining stability over 5 days of storage.

Conclusions:

The hollow-fibre column system is effective for preparing high-quality WPCs with minimal residual plasma proteins and high PLT recovery.
This system offers a valuable advancement in the preparation of washed platelets for clinical use.