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A hollow-fibre column system to effectively prepare washed platelets
S Tanaka1, T Hayashi1, S Sugaya2
1Japanese Red Cross Kinki Block Blood Centre, Ibaraki, Osaka, Japan.
Vox Sanguinis
|May 9, 2015
Summary
A new hollow-fibre column system effectively prepares washed platelet concentrates (WPCs), removing over 98% of plasma while maintaining high platelet recovery and function during storage.
Area of Science:
- Biomedical Engineering
- Hematology
- Transfusion Medicine
Background:
- Developing effective methods for preparing washed platelet concentrates (WPCs) is crucial for transfusion safety.
- Existing methods for washing platelets can be labor-intensive or may impact platelet viability.
Purpose of the Study:
- To evaluate the efficacy of a novel hollow-fibre column system for preparing WPCs.
- To assess the quality and in vitro characteristics of platelets processed by this system.
Main Methods:
- Quantified platelet (PLT) recovery and residual plasma proteins pre- and post-wash.
- Assessed in vitro PLT characteristics, including CD62P expression, aggregability, hypotonic shock response, and swirling scores, after 5 days of storage.
Main Results:
- The hollow-fibre system removed >98% of plasma with an average PLT recovery of 97%.
- Washed PLTs showed comparable or improved characteristics compared to pre-washed and manually washed platelets, maintaining stability over 5 days of storage.
Conclusions:
- The hollow-fibre column system is effective for preparing high-quality WPCs with minimal residual plasma proteins and high PLT recovery.
- This system offers a valuable advancement in the preparation of washed platelets for clinical use.
