Inducible and combinatorial gene manipulation in mouse brain
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Updated: Apr 12, 2026

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord
Published on: March 18, 2013
Godwin K Dogbevia1, Ricardo Marticorena-Alvarez2, Melanie Bausen3
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research Heidelberg, Germany ; Institute of Experimental and Clinical Pharmacology and Toxicology, University of Lübeck Lübeck, Germany.
Abstract:
We have deployed recombinant adeno-associated viruses equipped with tetracycline-controlled genetic switches to manipulate gene expression in mouse brain. Here, we show a combinatorial genetic approach for inducible, cell type-specific gene expression and Cre/loxP mediated gene recombination in different brain regions. Our chemical-genetic approach will help to investigate 'when', 'where', and 'how' gene(s) control neuronal circuit dynamics, and organize, for example, sensory signal processing, learning and memory, and behavior.

