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Inactivation of model viruses suspended in fresh frozen plasma using novel methylene blue based device
Ameneh Elikaei1, Zohreh Sharifi2, Seyed Masoud Hosseini1
1Department of Microbiology, Faculty of Biological Science ShahidBeheshti University GC,Tehran, Iran.
Background And Objective:
There is a concern on safety of human Fresh Frozen Plasma (FFP) as it is a source of some medicinal products. The possibility of transmission of blood-borne are reported often due to emerging viruses. There are some Pathogen Reduction Technologies (PRT) to inactivate viruses. Methylene Blue (MB) based method is one of them. The aim of this study was to examine new designated device to inactivate model viruses.
Materials And Methods:
Four model viruses were used in this study:Vesicular stomatitis virus (VSV), Herpes Simplex Virus I (HSV-1), Bovine Viral DiarrheaVirus(BVDV) and Polio Virus.50% Tissue Culture Infective Dose (TCID 50) and Reed-Muench Methods were used to titer the viruses. MB in two final concentration of 0.1 μM and 1 μM and illumination in about 627nm with red LED (Lamp Emitting Diode) for 15, 30, 45 and 60 minutes were used. Three replicates employed for each experiments.
Results:
1μMconcentration of MB showed more effective than 0.1μMin all designed illumination period for inactivation of HSV, VSV and BVDV. This method also demonstrated best results for enveloped model viruses. The most Log reduction for HSV, VSV and BVDV were6.28, 5.54 and 6.22, respectively. For HSV and BVDV inactivation, the best illumination period was 45 minutes.
Conclusion:
Model viruses showed sensitivity combination of MB and illumination using red LEDs. As results show this device could inactivate model viruses and reduce their titer very close to approved commercial devices, in compare.
Insights
A new device using Methylene Blue (MB) and red LED illumination effectively inactivates model viruses in plasma. This Methylene Blue based method shows significant log reduction, offering a promising approach for pathogen reduction technology.
Area of Science:
- Biotechnology
- Virology
- Blood Product Safety
Background:
- Fresh Frozen Plasma (FFP) is a source for medicinal products, raising safety concerns due to potential blood-borne virus transmission.
- Emerging viruses pose a continuous threat, necessitating robust pathogen reduction technologies (PRT).
- Methylene Blue (MB) based methods represent one such PRT for viral inactivation.
Purpose of the Study:
- To evaluate a novel device designed for inactivating model viruses using Methylene Blue (MB) and light.
- To assess the efficacy of MB and red LED illumination in reducing viral titers.
Main Methods:
- Four model viruses (VSV, HSV-1, BVDV, Polio Virus) were tested.
- Viral titers were determined using TCID50 and Reed-Muench methods.
- Inactivation was performed using MB at 0.1 μM and 1 μM concentrations with red LED illumination (627nm) for 15-60 minutes.
Main Results:
- 1 μM MB concentration was more effective than 0.1 μM for inactivating HSV, VSV, and BVDV across all illumination times.
- The method showed best results for enveloped model viruses, achieving log reductions of 6.28 (HSV), 5.54 (VSV), and 6.22 (BVDV).
- Optimal inactivation for HSV and BVDV was achieved with 45 minutes of illumination.
Conclusions:
- Model viruses demonstrated sensitivity to the combined Methylene Blue and red LED illumination.
- The developed device effectively inactivated model viruses, significantly reducing their titers.
- This technology shows comparable efficacy to approved commercial pathogen reduction devices.
