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On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
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Recent progress in developing proximity ligation assays for pathogen detection.

Christina Greenwood1, Gemma Johnson, Harvinder S Dhillon

  • 1Postgraduate Medical Institute, Faculty of Medical Science, Anglia Ruskin University, Chelmsford, Essex, UK.

Expert Review of Molecular Diagnostics
|May 9, 2015
PubMed
Summary
This summary is machine-generated.

Proximity assays offer sensitive and specific detection of infectious agents by combining immunoassays and nucleic acid tests (NATs). Further development is needed to overcome challenges for earlier and more accurate pathogen diagnosis.

Keywords:
antibodiesdiagnosticsdigital PCRhybridizationimmunoassaysproximity extension assayproximity ligation assayreal-time PCR• pathogens

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Area of Science:

  • Microbiology
  • Immunology
  • Molecular Biology

Background:

  • Effective infectious disease management relies on early pathogen detection.
  • Current immunodiagnostic tests lack sensitivity for early diagnosis.
  • Nucleic acid-based tests (NATs) are sensitive but don't confirm viable pathogens.

Purpose of the Study:

  • To explore the potential of proximity assays for earlier and more clinically relevant pathogen detection.
  • To address limitations of current diagnostic methods like immunoassays and NATs.

Main Methods:

  • Proximity assays combine antibody-based protein detection with NAT sensitivity.
  • Investigating antibody selection and combinations for assay optimization.
  • Addressing non-specific interactions and incubation times.

Main Results:

  • Proximity assays show promise for enhanced sensitivity and specificity in pathogen detection.
  • Challenges include identifying optimal antibody pairs and reducing assay times.
  • Potential for earlier and more accurate diagnosis compared to existing methods.

Conclusions:

  • Proximity assays represent a promising advancement in infectious disease diagnostics.
  • Overcoming technical hurdles is crucial to fully realize their potential.
  • Further research can lead to improved early detection and management of infections.