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High-risk HPV detection and genotyping by APTIMA HPV using cervical samples.
Michael T Pyne1, Camille L Hamula2, Keith Tardif1
1ARUP Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA.
Journal of Virological Methods
|May 10, 2015
Summary
High-risk human papillomavirus (HPV) testing is crucial for cervical cancer screening. Two HPV tests showed similar detection rates and high agreement, with a slight trend favoring one for HPV16 detection.
Area of Science:
- Gynecologic Oncology
- Virology
- Molecular Diagnostics
Background:
- Cervical cancer screening relies heavily on detecting high-risk human papillomavirus (HPV).
- Accurate HPV detection and genotyping are essential for effective screening strategies.
- Various molecular assays are available for HPV testing, necessitating comparative studies.
Purpose of the Study:
- To compare the performance of two HPV detection assays, HC2 and APTIMA HPV (AHPV), in cervical cytology specimens.
- To evaluate the agreement and positivity rates between the two HPV testing methods.
- To assess HPV16 positivity rates between the Cobas HPV test and the AHPV genotyping assay.
Main Methods:
- Analysis of 967 cervical cytology specimens preserved in PreservCyt.
- Comparison of HPV positivity rates between HC2 and AHPV tests.
- Assessment of overall agreement using Cohen's kappa coefficient (κ).
- Evaluation of HPV16 positivity trends between Cobas HPV and AHPV assays.
- A challenge experiment to detect cross-contamination with AHPV.
Main Results:
- Similar HPV positivity rates were observed for HC2 (13.8%) and AHPV (13.5%), with high overall agreement (94.6%, κ=0.77).
- A non-significant trend indicated higher HPV16 positivity with the Cobas HPV test (23.0%) compared to AHPV (19.5%).
- No cross-contamination was detected using the AHPV assay in the challenge experiment.
Conclusions:
- The HC2 and AHPV tests demonstrate comparable performance for high-risk HPV detection in cervical cytology specimens.
- The high agreement suggests both assays are reliable for cervical cancer screening.
- Further investigation may be warranted to clarify HPV16 genotyping differences between specific assays.
Keywords:
HPV
