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Purification and characterization of rabbit tissue factor
Anti-human tissue factor IgG column was used to partially purify rabbit tissue factor. The rabbit tissue factor was then further purified on SDS-polyacrylamide gel electrophoresis to prepare an essentially homogeneous rabbit tissue factor. This was used as an antigen to raise a high titer, monospecific, polyclonal antiserum for rabbit tissue factor. Immunostaining of a crude Triton extract of rabbit brain acetone powder and purified tissue factor with the anti-rabbit tissue factor IgG yielded a single major band with an apparent molecular weight of 45 kD, which corresponds to the mobility on SDS-PAGE of purified rabbit tissue factor apoprotein. The anti-rabbit tissue factor IgG was coupled to Affi-Gel-15 and used as an immunoadsorbent column to purify large amounts of rabbit brain tissue factor in a rapid, one-step technique.
Anti-human tissue factor IgG column was used to partially purify rabbit tissue factor. The rabbit tissue factor was then further purified on SDS-polyacrylamide gel electrophoresis to prepare an essentially homogeneous rabbit tissue factor. This was used as an antigen to raise a high titer, monospecific, polyclonal antiserum for rabbit tissue factor. Immunostaining of a crude Triton extract of rabbit brain acetone powder and purified tissue factor with the anti-rabbit tissue factor IgG yielded a single major band with an apparent molecular weight of 45 kD, which corresponds to the mobility on SDS-PAGE of purified rabbit tissue factor apoprotein. The anti-rabbit tissue factor IgG was coupled to Affi-Gel-15 and used as an immunoadsorbent column to purify large amounts of rabbit brain tissue factor in a rapid, one-step technique.
