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TM7 detection in human microbiome: Are PCR primers and FISH probes specific enough?
Maria V Sizova1, Sebastian N Doerfert1, Ekaterina Gavrish1
1Department of Biology, Northeastern University, Boston, MA 02115, USA.
Journal of Microbiological Methods
|May 11, 2015
Abstract:
TM7 appears important and omnipresent because it is repeatedly detected by molecular techniques in diverse environments. Here we report that most of primers and FISH probes thought to be TM7-specific do hybridize with multiple species from oral and vaginal cavity. This calls for re-examination of TM7 distribution and abundance.
