Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

16.4K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
16.4K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

4.7K
4.7K
Homologous Recombination02:31

Homologous Recombination

65.9K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
65.9K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

10.4K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.4K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

3.4K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.4K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

9.0K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
9.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Generalist biological artificial intelligence in modeling the language of life.

Nature biotechnology·2026
Same author

Virtual Cell Challenge: Toward a Turing test for the virtual cell.

Cell·2025
Same author

The Good and Bad of RNA:DNA Hybrids in Double-Strand Break Repair.

Molecular cell·2016
Same author

CRISPR-Mediated Base Editing without DNA Double-Strand Breaks.

Molecular cell·2016
Same author

Replication Origin Specification Gets a Push.

Molecular cell·2015
Same author

More division of labor at the eukaryotic replication fork.

Molecular cell·2014

Related Experiment Video

Updated: Apr 12, 2026

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks
09:07

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks

Published on: September 20, 2021

3.2K

Regulating transcription traffic around DSBs.

Brian S Plosky1

  • 1Molecular Cell, Cell Press, 600 Technology Square, 5(th) Floor, Cambridge, MA 02139, USA.

Molecular Cell
|May 11, 2015
PubMed
Summary

DNA double-strand breaks (DSBs) trigger mechanisms to halt transcription. Ui et al. reveal a link between elongation factors and repressive complexes to safeguard DNA integrity during repair.

Area of Science:

  • Molecular biology
  • Genetics
  • Cellular mechanisms

Background:

  • DNA double-strand breaks (DSBs) are severe DNA lesions.
  • Transcription can interfere with DNA repair processes.
  • Maintaining genomic stability is crucial for cell survival.

Purpose of the Study:

  • To investigate how cells prevent transcription near DSBs.
  • To identify factors involved in repressing transcription during DNA repair.

Main Methods:

  • The study likely involved molecular biology techniques.
  • Investigated protein-protein interactions.
  • Assessed the impact of DSBs on transcription.

Main Results:

  • A connection was found between an elongation factor and a repressive complex.

More Related Videos

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
10:44

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

10.9K
Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

9.8K

Related Experiment Videos

Last Updated: Apr 12, 2026

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks
09:07

Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks

Published on: September 20, 2021

3.2K
Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
10:44

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

10.9K
Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

9.8K
  • This complex prevents RNA polymerase activity near DSBs.

    • Conclusions:

    • Cells possess mechanisms to suppress transcription at DSB sites.
    • This suppression is vital for efficient and accurate DNA repair.