Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Voltmeter01:18

Voltmeter

3.5K
A voltmeter is an electrical device that measures the potential difference or voltage between two points. It is connected in parallel with the circuit element it is measuring. A parallel connection is used because elements in parallel experience the same potential difference. The voltmeter is represented by the symbol "V ".
An ideal voltmeter would have infinite resistance, so connecting it between two points in a circuit would not alter any of the currents. Real voltmeters always have...
3.5K
Voltammetric Techniques: Pulse Voltammetry01:17

Voltammetric Techniques: Pulse Voltammetry

2.0K
Differential-pulse voltammetry (DPV) is a type of voltammetry that involves applying a series of voltage pulses to an electrochemical cell while measuring the resulting current. In DPV, the differential pulse or small potential pulses are superimposed on a linear potential sweep. The magnitude of these pulses is typically small, often in the millivolt range. Each voltage pulse lasts a short duration, usually in the order of a few milliseconds, and is applied at regular intervals along the...
2.0K
Voltammetric Techniques: Linear-Scan (E vs Time)01:12

Voltammetric Techniques: Linear-Scan (E vs Time)

1.7K
Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...
1.7K
Voltammetry: Overview01:20

Voltammetry: Overview

3.4K
Voltammetry is an electroanalytical technique in which the current flowing through an electrochemical cell is measured as a function of applied potential, typically under conditions of concentration polarization. The technique provides valuable information about redox-active species, and the current response is plotted as a voltammogram.
A voltammetric cell uses three electrodes: a working electrode, a reference electrode, and an auxiliary electrode. The redox reactions occur in the working...
3.4K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

2.0K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
2.0K
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

3.6K
In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Two-Photon Polarization Ratio Explains Unusual Shape of the Two-Photon Absorption Spectra of Dyes and Detects Weak Excitonic Coupling in Fluorescent Protein Dimers.

The journal of physical chemistry. A·2026
Same author

Exploring 2-D σ-σ* Conjugation in Cyclic Polysiloxane Copolymers.

Macromolecular rapid communications·2026
Same author

ECgo: All-optical induction of single endothelial cell injury and capillary occlusion in the brain.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Reference standards for two-photon absorption cross-section and polarization ratio in the 440-630 nm wavelength range.

Optics express·2026
Same author

An Ultraresponsive Green Biosensor for Robust in Vivo Imaging of Synaptic Zinc Dynamics.

ACS sensors·2026
Same author

Human radical S-adenosylmethionine domain-containing 1 (RSAD1) is a Heme-binding protein.

Journal of inorganic biochemistry·2026

Related Experiment Video

Updated: Apr 12, 2026

A Simple Approach to Perform TEER Measurements Using a Self-Made Volt-Amperemeter with Programmable Output Frequency
07:43

A Simple Approach to Perform TEER Measurements Using a Self-Made Volt-Amperemeter with Programmable Output Frequency

Published on: October 5, 2019

24.1K

Two-photon voltmeter for measuring a molecular electric field.

Aleksander Rebane1,2, Geoffrey Wicks3, Mikhail Drobizhev3

  • 1Deptartment of Physics, Montana State University, 264 EPS, Bozeman, MT 59717 (USA). rebane@physics.montana.edu.

Angewandte Chemie (International Ed. in English)
|May 12, 2015
PubMed
Summary

This study introduces a novel method using light absorption measurements to determine solute-solvent interactions and molecular properties. The findings reveal a broad range for the reaction field and unique dielectric behavior at close molecular proximity.

Keywords:
intramolecular charge transfermolecular reaction fieldsolvatochromismsolvent effectstwo-photon absorption spectroscopy

More Related Videos

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
08:39

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: December 22, 2020

4.8K
Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization
08:22

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization

Published on: August 6, 2018

7.5K

Related Experiment Videos

Last Updated: Apr 12, 2026

A Simple Approach to Perform TEER Measurements Using a Self-Made Volt-Amperemeter with Programmable Output Frequency
07:43

A Simple Approach to Perform TEER Measurements Using a Self-Made Volt-Amperemeter with Programmable Output Frequency

Published on: October 5, 2019

24.1K
Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
08:39

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: December 22, 2020

4.8K
Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization
08:22

Measurement of Ultrafast Vibrational Coherences in Polyatomic Radical Cations with Strong-Field Adiabatic Ionization

Published on: August 6, 2018

7.5K

Area of Science:

  • Physical Chemistry
  • Spectroscopy
  • Computational Chemistry

Background:

  • Understanding solute-solvent interactions is crucial for predicting molecular behavior in solution.
  • Accurate determination of molecular properties like dipole moments and polarizability is essential for chemical and biological applications.

Purpose of the Study:

  • To develop and validate a new experimental approach for characterizing the solute-induced reaction field.
  • To determine ground- and excited-state dipole moments and polarizability of solvated chromophores.
  • To investigate the relationship between the effective dielectric constant and bulk dielectric constant at the molecular level.

Main Methods:

  • Utilizing one-photon and two-photon absorption spectroscopy.
  • Applying the method to benchmark chromophores: N,N-dimethyl-6-propionyl-2-naphthylamine (prodan) and coumarin 153 (C153).
  • Testing in various toluene/dimethyl sulfoxide (DMSO) solvent mixtures.

Main Results:

  • The developed approach accurately determines the reaction field strength and molecular properties.
  • Experimental values show good agreement with literature data and quantum-chemical calculations.
  • The reaction field spans a wide range (0-10^7 V/cm).
  • A unique functional dependence of the effective dielectric constant on the bulk dielectric constant was observed at close proximity.

Conclusions:

  • The new spectroscopic method provides a robust way to study solute-solvent interactions.
  • The results offer novel insights into molecular interactions at the nanoscale.
  • The findings contribute to a deeper understanding of solvation effects on molecular properties.