A novel analysis method for lactate dehydrogenase activity in serum samples based on fluorescence capillary analysis

Area of Science:

• Analytical Chemistry
• Biochemistry
• Clinical Diagnostics

Background:

• Lactate dehydrogenase (LDH) is a key biomarker in various clinical conditions.
• Accurate and efficient quantification of LDH activity is crucial for diagnosis and monitoring.
• Existing methods may require larger sample volumes and complex procedures.

Purpose of the Study:

• To develop a sensitive and reliable method for quantifying lactate dehydrogenase (LDH) activity.
• To optimize conditions for micro-volume sample analysis using fluorescence capillary technology.
• To assess the method's performance characteristics, including sensitivity, precision, and recovery.

Main Methods:

• Utilized fluorescence capillary analysis technology for LDH quantification.
• Optimized reaction reagent composition (phosphate buffer, NADH, pyruvate) and standard preparation.
• Evaluated performance metrics: linear range, detection limit, precision, and recovery.

Main Results:

• Achieved a linear response for LDH from 50 to 1200 U L(-1) with a detection limit of 31 U L(-1).
• Demonstrated high precision (RSD: 2.1 - 2.2%) and excellent recovery (96 - 105%).
• Required minimal sample (2 μL) and reagent (16 μL) volumes per test.

Conclusions:

• The developed method offers high sensitivity, low sample/reagent consumption, and good reliability.
• The technique is suitable for miniaturization into a portable bedside instrument for clinical use.
• This fluorescence-based approach enhances the efficiency and accessibility of LDH activity testing.