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Updated: Apr 12, 2026

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
Published on: June 28, 2018
Determination of pyruvic acid concentration using a bioluminescence system from Photobacterium leiognathi
Guanhua Xuan1, Xiaodong Lu, Jingxue Wang
1Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China. snow@ouc.edu.cn.
Abstract:
A novel, highly sensitive and selective bacterial luminescence method for the detection of pyruvic acid (PA) is reported here. This method is based on a reaction system catalyzed by lactate dehydrogenase (LDH) with the bacterial luciferase-FMN:NADH oxidoreductase bioluminescence system in vitro. The reduced nicotinamide adenine dinucleotide (NADH) involved in the LDH reaction system could be quantitatively analyzed by the bioluminescence system. A good linear relationship between the luminescence intensity and pyruvic acid concentration was exhibited within the range of 0.00014-0.001 mol l(-1), and the pyruvic acid detection limit was found to be 8.537 × 10(-5) mol l(-1). This method was successfully applied to the detection of PA in quail serum with a good recovery of over 70%.

