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Multilocus sequence analysis (MLSA) in prokaryotic taxonomy.

Stefanie P Glaeser1, Peter Kämpfer1

  • 1Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.

Systematic and Applied Microbiology
|May 12, 2015
PubMed
Summary

Multilocus sequence analysis (MLSA) offers higher resolution for bacterial phylogenetics and species delineation than 16S rRNA gene studies. However, standardized MLSA protocols are lacking, necessitating a critical review of its current applications in prokaryotic taxonomy.

Keywords:
MLSAMLSTPhylogenyResolutionTaxonomy

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Area of Science:

  • Microbiology
  • Genomics
  • Taxonomy

Background:

  • Multilocus sequence analysis (MLSA) uses conserved 'housekeeping genes' for high-resolution phylogenetic analysis.
  • MLSA is proposed for bacterial species delineation, potentially replacing DNA-DNA hybridization (DDH).

Purpose of the Study:

  • To review the historical development and current applications of MLSA in prokaryotic taxonomy.
  • To critically evaluate the advantages and disadvantages of various MLSA approaches.
  • To provide a perspective on MLSA's future role in genome-based taxonomy.

Main Methods:

  • Review of historical development of MLSA.
  • Critical analysis of current MLSA applications in prokaryotic taxonomy.
  • Highlighting variations in gene selection, number, and sequence comparison methods.

Main Results:

  • MLSA provides higher phylogenetic resolution than 16S rRNA gene studies.
  • Significant variations exist in MLSA protocols, lacking standardization.
  • MLSA is widely accepted but lacks universally agreed-upon recommendations for its application.

Conclusions:

  • Standardized MLSA protocols are needed for consistent application in microbial taxonomy.
  • MLSA offers a valuable tool for bacterial species delineation and phylogenetic studies.
  • Future MLSA applications may integrate with genome-based analyses for enhanced taxonomic resolution.