Identification of T cell clones without the need for sequencing

Area of Science:

• Immunology
• Cell Biology
• Genetics

Background:

• Brainbow recombination fluorescent protein systems are valuable for fate and lineage tracking.
• T lymphocyte tracking is crucial for understanding immune responses.
• Current lineage tracking methods can be resource-intensive.

Purpose of the Study:

• To utilize the Confetti mouse for T lymphocyte cell lineage tracking.
• To establish flow cytometry as a viable method for phenotyping T cell clonotypes.
• To offer an alternative to single-cell sequencing for lineage analysis.

Main Methods:

• Employing a mouse model with a ubiquitously expressed Brainbow construct (Confetti mouse).
• Performing T lymphocyte cell lineage tracking using the Confetti mouse.
• Utilizing flow cytometry for identification and phenotyping of T lymphocyte clonotypes.

Main Results:

• Successful lineage tracking of T lymphocytes was achieved using the Confetti mouse.
• Antigen-specific T lymphocyte clonotypes were identified and phenotyped.
• Flow cytometry proved effective for clonotype analysis, offering an alternative to sequencing.

Conclusions:

• The Confetti mouse model facilitates efficient T lymphocyte lineage tracking.
• Flow cytometry is a practical and cost-effective method for phenotyping T cell clonotypes.
• This approach streamlines T lymphocyte lineage analysis, reducing reliance on complex sequencing techniques.