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Related Experiment Video

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3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells
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Electrospun type 1 collagen matrices preserving native ultrastructure using benign binary solvent for cardiac tissue

Anuradha Elamparithi1,2, Alan M Punnoose2, Sarah Kuruvilla2,3,4

  • 1a Human Genetics, Sri Ramachandra University , Porur, Chennai , India.

Artificial Cells, Nanomedicine, and Biotechnology
|May 12, 2015
PubMed
Summary

This study introduces a new, eco-friendly electrospinning method using acetic acid and DMSO to create collagen nanofibers. The resulting scaffolds maintain collagen

Keywords:
3D cell cultureScaffoldscardiomyocytesselectrospinning

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Electrospinning fabricates ultrafine fibrous scaffolds for mimicking cellular microenvironments.
  • Collagen is a preferred biopolymer due to its biocompatibility.
  • Previous collagen electrospinning methods used toxic solvents and compromised collagen integrity.

Purpose of the Study:

  • To develop a non-toxic, economical method for electrospinning collagen type 1 nanofibers.
  • To preserve the ultrastructural integrity of collagen during electrospinning.
  • To evaluate the biocompatibility and potential applications of the collagen scaffolds in tissue engineering.

Main Methods:

  • Collagen type 1 was dissolved using glacial acetic acid and dimethyl sulfoxide (DMSO).
  • Electrospinning was performed to generate collagen nanofibers.
  • Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used for structural analysis.
  • Biocompatibility was assessed using MTT assays and confocal microscopy with L6 myoblasts and primary neonatal rat ventricular cardiomyocytes (NRVCM).

Main Results:

  • The novel solvent system (glacial acetic acid and DMSO) successfully produced collagen type 1 nanofibers.
  • TEM confirmed the preservation of native collagen ultrastructure, including 67 nm D-periodicity banding.
  • SEM showed fiber diameters ranging from 200-1100 nm.
  • The 3D collagen scaffolds demonstrated excellent biocompatibility and supported the contractile function of NRVCM for 17 days.

Conclusions:

  • A novel, benign solvent system enables the electrospinning of collagen type 1 without copolymers, preserving its native ultrastructure.
  • The developed collagen scaffolds are non-toxic, economical, and biocompatible.
  • This method holds significant potential for applications in tissue engineering, particularly for cardiac and muscle tissue regeneration.