Related Experiment Video

Updated: Apr 12, 2026

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
09:40

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy

Published on: October 4, 2019

6.1K

miR-20a mediates temozolomide-resistance in glioblastoma cells via negatively regulating LRIG1 expression

Junhua Wei1, Xuchen Qi2, Qitao Zhan3

  • 1Department of Intensive Care Unit, Sir Run Run Shaw Hospital, College of Medical Sciences, Zhejiang University, Hangzhou 310016, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|May 12, 2015
PubMed
Abstract

Insights

MicroRNA-20a (miR-20a) promotes temozolomide resistance in glioblastoma by suppressing LRIG1. Inhibiting miR-20a or restoring LRIG1 may overcome drug resistance in glioma patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Temozolomide (TMZ) resistance is a significant challenge in treating glioblastoma multiforme (GBM).
  • MicroRNAs (miRNAs) play a crucial role in modulating drug resistance across various cancers.
  • Understanding the specific miRNAs involved in TMZ resistance in GBM is vital for developing effective therapies.

Purpose of the Study:

  • To investigate the role of miR-20a in temozolomide (TMZ) resistance in glioblastoma (GBM).
  • To identify the relationship between miR-20a, its target gene LRIG1, and TMZ resistance in GBM.
  • To explore the potential of targeting miR-20a and LRIG1 for glioma treatment.

Main Methods:

  • Quantitative real-time PCR and western blot were used to assess miR-20a and LRIG1 expression levels.
  • Cell viability assays were performed to determine the sensitivity of GBM cells to TMZ.
  • MiR-20a levels were modulated using inhibitors and mimics, and LRIG1 was overexpressed via pcDNA-LRIG1.
  • A 3'-UTR dual-luciferase reporter assay confirmed the interaction between miR-20a and LRIG1.
  • In vivo studies utilized a xenograft tumor model to evaluate antitumor activity.

Main Results:

  • Glioblastoma cells resistant to TMZ (U251/TMZ) exhibited high miR-20a and low LRIG1 expression.
  • Inhibiting miR-20a restored TMZ sensitivity in GBM cells, while miR-20a overexpression increased resistance.
  • LRIG1 expression was inversely correlated with miR-20a levels, and miR-20a directly targeted LRIG1's 3'UTR.
  • Knockdown of LRIG1 reversed the effect of miR-20a inhibition, confirming its role in mediating resistance.

Conclusions:

  • MiR-20a contributes to TMZ resistance in glioblastoma by negatively regulating LRIG1 expression.
  • The miR-20a/LRIG1 axis represents a potential therapeutic target for overcoming drug resistance in glioma.
  • Modulating miR-20a and LRIG1 offers a promising strategy for enhancing the efficacy of TMZ treatment in GBM.
Keywords:
Glioblastoma cellsLeucine-rich repeats and immunoglobulin-like domains 1 (LRIG1)TemozolomidemiR-20a

More Related Videos

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.1K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.8K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.1K