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Related Concept Videos

2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

817
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
817
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

1.4K
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
1.4K
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

1.8K
The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
1.8K
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

1.8K
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
1.8K
¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

2.2K
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
2.2K
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

3.9K
The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved...
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Related Experiment Video

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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

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RNA structure determination by solid-state NMR spectroscopy.

Alexander Marchanka1, Bernd Simon1, Gerhard Althoff-Ospelt2

  • 1Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.

Nature Communications
|May 12, 2015
PubMed
Summary
This summary is machine-generated.

Solid-state NMR spectroscopy provides high-resolution RNA structure determination, overcoming limitations of crystallization and solution-state NMR. This advance enables structural studies of large RNA-protein complexes, crucial for understanding cellular mechanisms.

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Area of Science:

  • Structural Biology
  • Biochemistry
  • Molecular Biophysics

Background:

  • Understanding RNA's three-dimensional structure is vital for elucidating cellular processes.
  • RNA's flexibility poses challenges for traditional crystallization methods.
  • Solution-state Nuclear Magnetic Resonance (NMR) is limited for large, complex biological assemblies.

Purpose of the Study:

  • To present solid-state NMR spectroscopy as a novel approach for RNA structure determination.
  • To enable high-resolution structural analysis of RNA within large molecular machines.
  • To facilitate mechanistic studies of RNA-protein assemblies that are difficult to analyze.

Main Methods:

  • Development of specialized solid-state NMR experiments.
  • Implementation of novel RNA labeling strategies.
  • Application of solid-state NMR to analyze RNA structure.

Main Results:

  • Demonstration of high-resolution RNA structure determination using solid-state NMR for the first time.
  • Successful structural analysis of segmentally labeled RNA within high-molecular-weight complexes.
  • Methodology is independent of the sample's ability to crystallize.

Conclusions:

  • Solid-state NMR spectroscopy is a powerful technique for high-resolution RNA structural analysis.
  • This method overcomes limitations of crystallization and solution-state NMR for complex systems.
  • Opens new avenues for studying the structure and function of challenging RNA-protein assemblies.