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15N Hyperpolarization by Reversible Exchange Using SABRE-SHEATH.

Milton L Truong1, Thomas Theis2, Aaron M Coffey1

  • 1Institute of Imaging Science, Department of Radiology, Department of Biomedical Engineering, Department of Physics and Astronomy, Department of Biochemistry, and Vanderbilt-Ingram Cancer Center (VICC), Vanderbilt University , Nashville, Tennessee 37232-2310, United States.

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This summary is machine-generated.

NMR signal amplification by reversible exchange (SABRE) hyperpolarization in microtesla fields enables 15N MRI. This technique enhances 15N polarization for advanced biomedical imaging and spectroscopy applications.

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Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Hyperpolarization Techniques
  • Biomedical Imaging

Background:

  • NMR signal amplification by reversible exchange (SABRE) enhances molecular polarization using parahydrogen and iridium catalysts.
  • Previous work demonstrated 10% 15N-polarization using SABRE in microtesla fields.
  • 15N hyperpolarization offers advantages like longer signal lifetime and wider chemical shift dispersion compared to proton hyperpolarization.

Purpose of the Study:

  • To demonstrate 15N magnetic resonance imaging (MRI) enabled by high 15N-polarization levels.
  • To develop a theoretical model for hyperpolarization transfer to heteronuclei.
  • To identify key parameters for optimizing 15N-hyperpolarization efficiency.

Main Methods:

  • SABRE hyperpolarization performed in microtesla fields within a magnetic shield.
  • Systematic study of parahydrogen pressure, flow rate, temperature, catalyst-to-substrate ratio, and oxygen quenching effects.
  • Demonstration of 15N-hyperpolarized MRI and proof-of-principle 13C-hyperpolarization.

Main Results:

  • Achieved unprecedented 15N-polarization levels, enabling 15N MRI with 2x2 mm2 resolution.
  • Presented a theoretical model for heteronuclei hyperpolarization transfer.
  • Identified critical parameters influencing 15N-hyperpolarization efficiency.
  • Successfully demonstrated 13C-hyperpolarization using the SABRE method.

Conclusions:

  • High 15N-polarization via SABRE in microtesla fields is sufficient for 15N MRI.
  • The developed method is simple, requiring only parahydrogen and a magnetic shield.
  • This technique enables hyperpolarization of molecules with NMR T1 relaxation times suitable for biomedical applications.