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Updated: Apr 12, 2026

Visualization of Organelles In Situ by Cryo-STEM Tomography
Published on: June 23, 2023
Structural analysis of multicellular organisms with cryo-electron tomography
Jan Harapin1, Mandy Börmel2, K Tanuj Sapra1
1Department of Biochemistry, University of Zurich, Zurich, Switzerland.
Abstract:
We developed a method for visualizing tissues from multicellular organisms using cryo-electron tomography. Our protocol involves vitrifying samples with high-pressure freezing, thinning them with cryo-FIB-SEM (focused-ion-beam scanning electron microscopy) and applying fiducial gold markers under cryogenic conditions to the lamellae post-milling. We applied this protocol to acquire tomograms of vitrified Caenorhabditis elegans embryos and worms, which showed the intracellular organization of selected tissues at particular developmental stages in otherwise intact specimens.
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