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Updated: Apr 12, 2026

Purification of Hsp104, a Protein Disaggregase
Published on: September 30, 2011
Rescue of αB Crystallin (HSPB5) Mutants Associated Protein Aggregation by Co-Expression of HSPB5 Partners
Rasha M Hussein1, Ivor J Benjamin2, Harm H Kampinga3
1Department of Cell Biology, University Medical Center Groningen, Groningen, The Netherlands; Department of Biochemistry, Faculty of Pharmacy, Beni-Suef University, Salah Salem Street, 62511, Beni-Suef, Egypt.
Abstract:
HSPB5 (also called αB-crystallin) is a ubiquitously expressed small heat shock protein. Mutations in HSPB5 have been found to cause cataract, but are also associated with a subgroup of myofibrillar myopathies. Cells expressing each of these HSPB5 mutants are characterized by the appearance of protein aggregates of primarily the mutant HSPB5. Like several members of the HSPB family, HSPB5 can form both homo-oligomeric and hetero-oligomeric complexes. Previous studies showed that co-expression of HSPB1 and HSPB8 can prevent the aggregation associated with the HSPB5 (R120G) mutant in cardiomyocytes and in transgenic mice. In this study, we systematically compared the effect of co-expression of each of the members of the human HSPB family (HSPB1-10) on the aggregation of three different HSPB5 mutants (R120G, 450 Δ A, 464 Δ CT). Of all members, co-expression of HSPB1, HSPB4 and HSPB5 itself, most effectively prevent the aggregation of these 3 HSPB5 mutants. HSPB6 and HSPB8 were also active but less, whilst the other 5 HSPB members were ineffective. Co-expression of Hsp70 did not reduce the aggregation of the HSPB5 mutants, suggesting that aggregate formation is most likely not related to a toxic gain of function of the mutants per se, but rather related to a loss of chaperone function of the oligomeric complexes containing the HSPB5 mutants (dominant negative effects). Our data suggest that the rescue of aggregation associated with the HSPB5 mutants is due to competitive incorporation of its partners into hetero-oligomers hereby negating the dominant negative effects of the mutant on the functioning of the hetero-oligomer.
Insights
Small heat shock proteins (HSPB) family members, particularly HSPB1, HSPB4, and HSPB5, can prevent harmful protein aggregation caused by HSPB5 mutations. This suggests a loss of chaperone function, not a toxic gain, is responsible for HSPB5-related diseases.
Area of Science:
- Biochemistry
- Molecular Biology
- Genetics
Background:
- Small heat shock proteins (HSPB) are crucial for cellular proteostasis.
- Mutations in HSPB5 (αB-crystallin) are linked to cataracts and myofibrillar myopathies, causing protein aggregation.
- HSPB5 mutations may exert dominant-negative effects due to impaired chaperone function.
Purpose of the Study:
- To systematically investigate the protective effects of co-expressing different HSPB family members against HSPB5 mutant aggregation.
- To elucidate the mechanism underlying HSPB5 mutant aggregation and potential therapeutic strategies.
Main Methods:
- Systematic co-expression of human HSPB family members (HSPB1-10) with three distinct HSPB5 mutants (R120G, 450 Δ A, 464 Δ CT).
- Assessment of protein aggregate formation in cellular models.
- Comparison of the efficacy of different HSPB members in preventing aggregation.
Main Results:
- Co-expression of HSPB1, HSPB4, and HSPB5 significantly reduced aggregation of all tested HSPB5 mutants.
- HSPB6 and HSPB8 showed moderate protective effects, while other HSPB members were ineffective.
- Hsp70 co-expression did not mitigate aggregation, supporting a loss-of-function mechanism for HSPB5 mutants.
Conclusions:
- HSPB1, HSPB4, and HSPB5 are potent suppressors of HSPB5 mutant aggregation.
- The protective mechanism involves competitive incorporation into hetero-oligomers, negating dominant-negative effects.
- Targeting HSPB interactions offers a potential therapeutic avenue for HSPB5-related disorders.
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