Related Experiment Video
Updated: Apr 12, 2026
08:00
Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
Published on: October 4, 2024
1.2K
Enzyme-Responsive Delivery of Multiple Proteins with Spatiotemporal Control
Suwei Zhu1, Lina Nih1, S Thomas Carmichael2
1Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, Los Angeles, CA, 90095, USA.
Advanced Materials (Deerfield Beach, Fla.)
|May 13, 2015
Summary
A new single-protein technology enables controlled delivery of therapeutic proteins at wound sites using natural enzymes. This approach demonstrated sustained and sequential protein delivery for enhanced stroke and skin wound healing.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Drug Delivery Systems
Background:
- Biological materials like enzymes and growth factors are crucial for tissue and organ development.
- Precise control over therapeutic protein delivery is essential for effective wound healing and tissue regeneration.
Purpose of the Study:
- To develop a novel chirality-controlled, single-protein technology for spatiotemporally defined therapeutic protein delivery.
- To investigate the potential of this technology for wound healing applications, specifically stroke and skin wounds.
Main Methods:
- Development of a single-protein technology leveraging chirality control.
- Utilizing natural enzymes present at wound sites to trigger protein release.
- Demonstrating sustained delivery of a single therapeutic protein.
- Demonstrating sequential delivery of two therapeutic proteins.
Main Results:
- The developed technology enables controlled release of therapeutic proteins.
- Sustained delivery of one protein was successfully achieved.
- Sequential delivery of two distinct proteins was demonstrated.
- The technology shows promise for applications in stroke and skin wound healing.
Conclusions:
- Chirality-controlled, single-protein technology offers a novel approach for precise therapeutic protein delivery.
- This technology can be engineered for enzyme-responsive release at wound sites.
- The demonstrated sustained and sequential delivery capabilities are significant for regenerative medicine and therapeutic applications.
More Related Videos
Related Concept Videos
01:33Overview of Secretory Vesicles
10.4K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
10.4K
01:34Directing Proteins to the Rough Endoplasmic Reticulum
18.4K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
18.4K
01:22Modified-Release Drug Delivery Systems: Rate-Programmed I
131
Rate-programmed drug delivery systems (DDS) are designed to release drugs at specific, controlled rates to maintain consistent therapeutic levels. These systems are categorized based on their release mechanisms, including dissolution-controlled DDS, diffusion-controlled DDS, and combined dissolution-diffusion-controlled DDS.In dissolution-controlled DDS, the release rate depends on the slow dissolution of the drug itself or the surrounding matrix. Drugs with inherently slow dissolution rates,...
131
01:45Regulation of Nuclear Protein Sorting
3.5K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.5K
01:19Protein Dynamics in Living Cells
2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K
01:30Modified-Release Drug Delivery Systems: Stimuli-Activated
139
Stimuli-activated drug delivery systems are designed to release drugs in response to specific physical, chemical, or biological stimuli. These systems often utilize hydrogels—three-dimensional, hydrophilic polymer networks capable of swelling in aqueous environments and retaining significant fluid volumes. Upon exposure to particular stimuli, these hydrogels undergo structural transitions that allow the embedded drug to be released. Due to this adaptive behavior, such systems are also...
139