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Establishment and characterization of triple drug resistant head and neck squamous cell carcinoma cell lines

Sindhu Valiyaveedan Govindan1, Safeena Kulsum1, Ramanan Somasundara Pandian2

  • 1Integrated Head and Neck Oncology Research Program, DSRG‑5, Mazumdar Shaw Centre for Translational Research, Mazumdar Shaw Medical Foundation, Narayana Health, Bangalore, Karnataka 560099, India.

Molecular Medicine Reports
|May 13, 2015
PubMed

Insights

Researchers developed triple drug-resistant head and neck cancer cell lines (TPFR) to study chemoresistance mechanisms. These models aid in understanding drug resistance and designing new cancer therapies.

Area of Science:

  • Oncology
  • Cancer Biology
  • Pharmacology

Background:

  • Chemotherapy resistance significantly impacts cancer patient outcomes, particularly in head and neck cancer.
  • Effective treatment strategies for head and neck cancers often involve chemotherapy, but response rates vary.
  • In vitro chemoresistant cell line models are crucial for investigating drug resistance mechanisms and developing new therapeutic agents.

Purpose of the Study:

  • To generate head and neck cancer cell lines resistant to a triple chemotherapy regimen (docetaxel, cisplatin, and 5-fluorouracil).
  • To establish valuable in vitro models for elucidating the mechanisms of chemoresistance.
  • To provide a resource for the development of novel anti-cancer drugs and treatment strategies.

Main Methods:

  • Two head and neck squamous cell carcinoma cell lines (Hep-2 and CAL-27) were used.
  • Sequential exposure to increasing concentrations of docetaxel, cisplatin, and 5-fluorouracil (TPF) was employed to induce triple drug resistance.
  • Generated resistant cell lines were designated Hep-2 TPFR and CAL-27 TPFR.
  • Analyses included cell viability, apoptosis, cell cycle progression, and expression of multidrug resistance genes.

Main Results:

  • Successfully generated triple drug-resistant head and neck cancer cell lines (Hep-2 TPFR and CAL-27 TPFR).
  • Characterization of these resistant cell lines revealed alterations in cell viability, apoptosis, cell cycle, and multidrug resistance gene expression compared to parental cells.
  • The study provides a foundation for further mechanistic studies into chemoresistance.

Conclusions:

  • The developed TPFR cell lines serve as a valuable tool for studying chemoresistance in head and neck cancer.
  • These models can facilitate the discovery of novel therapeutic targets and drug design for overcoming drug resistance.
  • Further research using these models is warranted to improve treatment outcomes for head and neck cancer patients.

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