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Updated: Aug 12, 2026
Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
Published on: November 28, 2015
Immunohistological investigation of mononuclear cell infiltrates in meningiomas
1Institut für Hirnforschung, Universität Tübingen, Federal Republic of Germany.
Abstract:
Immunohistochemical analysis of inflammatory cell density and infiltrate subpopulations in 42 meningiomas was performed. Evaluation of infiltrating cell density was carried out by cell counting. Meningothelial and fibroblastic meningiomas contained an average of 3% mononuclear cells; the few lymphocytes were localized in the perivascular spaces. In subtypes with cellular atypies and recurrent tumors, the inflammatory cells increased up to 9%. We found small mononuclear cell clusters in the tumor parenchyma in addition to the perivascular infiltrates. Marked degrees of infiltration were found in anaplastic meningiomas (average 13.5% of total cells). The lymphocytic infiltrates were localized in multilayered perivascular cuffings and intraparenchymal cell clusters. The composition of the infiltrates, i.e., predominantly a mixed staining of cytotoxic/suppressor and helper cell phenotypes, did not vary in the different subtypes. We conclude: (1) that inflammatory infiltration is more frequent and denser in malignant than in benign meningiomas; and (2) that the tumor defense mechanisms in meningiomas are mediated particularly by T cell mediated immunity.
Insights
Inflammatory cell infiltration is more pronounced in malignant meningiomas compared to benign types. Tumor defense mechanisms in meningiomas are primarily mediated by T-cell immunity.
Area of Science:
- Neuro-oncology
- Immunology
- Pathology
Background:
- Meningiomas are primary tumors of the central nervous system, with varying grades of malignancy.
- The role of inflammatory cells and their subtypes in meningioma progression is not fully understood.
Purpose of the Study:
- To investigate the density and subpopulations of inflammatory cells in different meningioma subtypes.
- To determine the relationship between inflammatory infiltration and meningioma malignancy.
Main Methods:
- Immunohistochemical analysis of 42 meningioma samples.
- Cell counting to evaluate inflammatory cell density and infiltrate composition.
Main Results:
- Benign meningiomas (meningothelial, fibroblastic) showed 3% mononuclear cells, primarily perivascular lymphocytes.
- Malignant subtypes with atypia and recurrent tumors had increased inflammatory cells (up to 9%).
- Anaplastic meningiomas exhibited marked infiltration (average 13.5%), with intraparenchymal clusters and perivascular cuffing.
Conclusions:
- Inflammatory infiltration is significantly more frequent and denser in malignant meningiomas than in benign ones.
- T-cell mediated immunity appears to be a key component of the tumor defense mechanisms in meningiomas.
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