Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapid and quantitative phage susceptibility test by ramanome.

mLife·2026
Same author

Harnessing CRISPR-Cas12 and Microfluidics Chips for Multiplex Respiratory Pathogens Diagnosis.

ACS sensors·2026
Same author

Smartphone-Based Microbubble-Linked Immunosorbent Assay Powered by Classification-Regression Integrated Deep Learning for Portable Quantitative Biomarker Analysis.

ACS nano·2026
Same author

Culture-Free Microfluidics for Ultra-Rapid Antimicrobial Susceptibility Testing with AI in Resource-Limited Settings.

Analytical chemistry·2026
Same author

Gravity-Driven Formation of Water-in-Wax Spheres for Efficient One-Pot CRISPR Diagnostics.

ACS nano·2026
Same author

AI-integrated smartphone platform enables POC dual-channel glucose monitoring with an indicator-free nanozyme gel kit.

Biosensors & bioelectronics·2026

Related Experiment Video

Updated: Apr 12, 2026

Microfluidic-based Electrotaxis for On-demand Quantitative Analysis of Caenorhabditis elegans' Locomotion
10:23

Microfluidic-based Electrotaxis for On-demand Quantitative Analysis of Caenorhabditis elegans' Locomotion

Published on: May 2, 2013

10.4K

Highly efficient microfluidic sorting device for synchronizing developmental stages of C. elegans based on deflecting

Xixian Wang1, Rui Hu, Anle Ge

  • 1Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. bfliu@mail.hust.edu.cn weidu@mail.hust.edu.cn.

Lab on a Chip
|May 13, 2015
PubMed
Summary

This study introduces a cost-effective microfluidic device for sorting Caenorhabditis elegans (C. elegans) by developmental stage using electrotaxis. This method efficiently separates worms, providing synchronized populations for biological research.

More Related Videos

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans
10:39

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

Published on: February 19, 2018

11.2K
A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans
10:45

A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans

Published on: April 11, 2022

2.6K

Related Experiment Videos

Last Updated: Apr 12, 2026

Microfluidic-based Electrotaxis for On-demand Quantitative Analysis of Caenorhabditis elegans' Locomotion
10:23

Microfluidic-based Electrotaxis for On-demand Quantitative Analysis of Caenorhabditis elegans' Locomotion

Published on: May 2, 2013

10.4K
Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans
10:39

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

Published on: February 19, 2018

11.2K
A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans
10:45

A Simple Microfluidic Chip for Long-Term Growth and Imaging of Caenorhabditis elegans

Published on: April 11, 2022

2.6K

Area of Science:

  • Developmental Biology
  • Microfluidics
  • Model Organisms

Background:

  • Caenorhabditis elegans (C. elegans) is a crucial model organism in biological research.
  • Synchronized populations of different developmental stages are essential for stage-specific studies.
  • Existing methods for C. elegans synchronization can be labor-intensive.

Purpose of the Study:

  • To develop a novel, cost-effective microfluidic device for simultaneous sorting of C. elegans by developmental stage.
  • To utilize the phenomenon of deflecting electrotaxis for efficient worm separation.
  • To provide a versatile platform for obtaining stage-synchronized C. elegans populations.

Main Methods:

  • Fabrication of a microfluidic device using polydimethylsiloxane (PDMS) with angled sorting channels.
  • Hybridization of the microfluidic device to an agarose plate.
  • Application of an electric field to induce deflecting electrotaxis for worm sorting.
  • Utilizing lon-2 mutant worms to investigate the role of body size in electrotactic response.

Main Results:

  • The microfluidic device successfully sorted C. elegans into different developmental stages based on their deflecting electrotaxis.
  • Different stages of C. elegans exhibited distinct crawling angles towards the negative electrode.
  • Body size was identified as a key factor influencing the deflecting angle in adult worms.
  • The device demonstrated efficacy in sorting wild-type hermaphrodites, mutants, and males.

Conclusions:

  • The developed microfluidic device offers a versatile and efficient platform for obtaining large numbers of stage-synchronized C. elegans.
  • This technology facilitates research requiring specific developmental stages of C. elegans.
  • The device has potential applications in studying the neuronal basis of deflecting electrotaxis.