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Updated: Apr 12, 2026

Pull-down of Calmodulin-binding Proteins
Published on: January 23, 2012
Molecular mechanisms of protein kinase regulation by calcium/calmodulin
Bertrand Simon1, Anne-Sophie Huart1, Matthias Wilmanns1
1EMBL Hamburg, c/o DESY, Building 25A, Notkestraße 85, 22603 Hamburg, Germany.
Abstract:
Many human protein kinases are regulated by the calcium-sensor protein calmodulin, which binds to a short flexible segment C-terminal to the enzyme's catalytic kinase domain. Our understanding of the molecular mechanism of kinase activity regulation by calcium/calmodulin has been advanced by the structures of two protein kinases-calmodulin kinase II and death-associated protein kinase 1-bound to calcium/calmodulin. Comparison of these two structures reveals a surprising level of diversity in the overall kinase-calcium/calmodulin arrangement and functional readout of activity, as well as complementary mechanisms of kinase regulation such as phosphorylation.
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