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Updated: Apr 12, 2026
Isolation and Characterization of Neutrophils with Anti-Tumor Properties
Published on: June 19, 2015
Uniform Combretastatin-induced Effect on Monocytes and Neutrophils in Peripheral Blood but Not in Tumors
Anja B Bohn1, Anne S Brems-Eskildsen2, Bjarne K Møller3
1Department of Experimental Clinical Oncology, Aarhus University Hospital, Aarhus, Denmark Department of Clinical Immunology, Aarhus University Hospital, Aarhus, Denmark anjsch@rm.dk.
Background/Aim:
Combretastatin A-4 phosphate (CA4P) is a vascular-disrupting agent which affects the level of circulating neutrophils. Since tumor-associated macrophages and neutrophils may collaborate, we compared the effect of CA4P treatment on monocytes/macrophages and neutrophils.
Material And Methods:
CDF1 mice with a C3H mammary carcinoma foot tumor were injected intraperitoneally with CA4P. Blood samples were taken and tumors excised at various time-points after treatment. Circulating monocytes and granulocytes were detected by flow cytometry and the tumor levels of these cell types was estimated immunohistochemically.
Results:
CA4P induced similar oscillating effects on the level of circulating monocytes and of neutrophils, with an initial decrease followed by an increase and a return to control levels at 6-h and 24-h, respectively. In tumors, only the macrophage level decreased significantly after treatment.
Conclusion:
CA4P induced similar changes in the level of circulating monocytes and neutrophils, but only affected the fraction of macrophages significantly.
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