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Expression of miRNAs as Important Element of Melanoma Cell Plasticity in Response to Microenvironmental Stimuli
Michal Wozniak1, Malgorzata Sztiller-Sikorska2, Malgorzata Czyz2
1Department of Molecular Biology of Cancer, Medical University of Lodz, Lodz, Poland michal.wozniak@umed.lodz.pl.
Background:
Melanoma cells form monolayers in serum-containing media, however, in serum-free media they form anchorage-independent spheroids. We investigated miRNAs differentially expressed between these culture types and identified those that possibly control the plasticity of melanoma cells.
Materials And Methods:
The expression of miRNAs in melanoma cells was evaluated with microarrays, and certain miRNAs were validated with real-time PCR. Several bioinformatic tools were used to assess the involvement of identified miRNAs in cancer-related pathways, and to compile the results of mRNA microarray data from the same melanoma cells.
Results:
A total of 19 miRNAs were differentially expressed between monolayers and spheroids. miRNAs up-regulated in spheroids modulated cell motility and migration, whereas those up-regulated in monolayers suppressed melanogenesis.
Conclusion:
The present study identified those miRNAs that participate in the regulation of melanoma cell plasticity.
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