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Simultaneous determination of centchroman and tamoxifen along with their metabolites in rat plasma using LC-MS/MS
Kanumuri Siva Rama Raju1, Isha Taneja, Sheelendra Pratap Singh
1Academy of Scientific & Innovative Research, New Delhi, India.
Aim:
Tamoxifen and centchroman are two non-steroidal, selective estrogen receptors modulators, intended for long term therapy in the woman. Because of their wide spread use, there is a possibility of co-prescription of these agents.
Materials & Methods:
We studied the probable pharmacokinetic interaction between these agents in breast cancer model rats. A simple, sensitive and rapid LC-ESI-MS/MS method was developed and validated for the simultaneous determination of tamoxifen, centchroman and their active metabolites.
Results:
The method was linear over a range of 0.2-200 ng/ml. All validation parameters met the acceptance criteria according to regulatory guidelines.
Conclusion:
LC-MS/MS method for determination of tamoxifen, centchroman and their metabolites was developed and validated. Results show the potential of drug-drug interaction upon co-administration these two marketed drugs.
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