Expression and Purification of RNA-Protein Complexes in Escherichia coli
Margueritte El Khouri1, Marjorie Catala, Bili Seijo
1Center for Molecular OncologyBarts Cancer Institute, Queen Mary University of London, London, UK.
Methods in Molecular Biology (Clifton, N.J.)
|May 14, 2015
Abstract:
For structural, biochemical or pharmacological studies, it is required to have pure RNA in large quantities. We previously devised a generic approach that allows efficient in vivo expression of recombinant RNA in Escherichia coli. We have extended the "tRNA scaffold" method to RNA/protein co-expression in order to express and purify RNA by affinity in native condition. As a proof-of-concept, we present the expression and the purification of the AtRNA-mala in complex with the MS2 coat protein.
