Related Experiment Video
Updated: Apr 12, 2026

Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research
Published on: September 27, 2024
Raman spectroscopic characterisation of resin-infiltrated hypomineralised enamel
Arun K Natarajan1, Sara J Fraser, Michael V Swain
1Department of Oral Sciences, Faculty of Dentistry, University of Otago, PO Box 647, Dunedin, 9054, New Zealand.
Abstract:
Raman spectroscopy was used to investigate how the effect of pre-treatment protocols, with combinations of hydrochloric acid (HCl), sodium hypochlorite (NaOCl) and hydrogen peroxide (H2O2), for molar-incisor hypo-mineralisation (MIH) altered the penetration depth of polymer infiltrants (ICON, DMG, Hamburg, Germany). Furthermore, the effect on the structure of the MIH portions of the teeth with treatment is examined using multivariate analysis of spectra. It was found that pre-treatment protocols improved penetration depths. The structure of the MIH portion post-treatment appeared much closer to that of normal enamel suggesting a diminution of protein in the MIH region with treatment.
Related Concept Videos
Raman Spectroscopy: Overview
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
Raman Spectroscopy Instrumentation: Overview
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

