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Updated: Apr 12, 2026
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The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
Published on: February 28, 2017
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Calcitonin-induced effects on amniotic fluid-derived mesenchymal stem cells
Caterina Morabito1, Iolanda D'Alimonte, Laura Pierdomenico
1Department of Neuroscience, Imaging and Clinical Sciences, 'G. d'Annunzio' University of Chieti- Pescara, Chieti, Italy.
Summary
Calcitonin enhances osteogenic differentiation in human amniotic fluid-derived mesenchymal stem cells (huAFMSCs). This study reveals calcitonin
Area of Science:
- Stem cell biology
- Bone biology
- Endocrinology
Background:
- Human amniotic fluid-derived mesenchymal stem cells (huAFMSCs) possess multi-lineage differentiation potential and lack tumorigenicity.
- Mesenchymal stem cells are promising for therapeutic applications.
- Calcitonin plays a role in bone homeostasis.
Purpose of the Study:
- To investigate the effects of calcitonin on huAFMSCs during osteogenic differentiation.
- To elucidate the physiological role of calcitonin in bone homeostasis using huAFMSCs.
Main Methods:
- Assayed calcitonin receptor expression in huAFMSCs using immunolabelling.
- Measured huAFMSC proliferation and osteogenesis via colorimetric and enzymatic assays.
- Quantified intracellular Ca(2+) and cAMP levels using videomicroscopy and spectrophotometry.
Main Results:
- Calcitonin receptor was expressed in proliferating and osteo-differentiated huAFMSCs.
- Calcitonin stimulated intracellular Ca(2+) and cAMP production.
- Calcitonin inhibited proliferation and promoted osteogenic differentiation in a dose-dependent manner, indicated by enhanced markers and alkaline phosphatase activity.
Conclusions:
- huAFMSCs serve as a viable in-vitro model for studying cellular responses to calcitonin.
- Findings suggest new avenues for research in cell therapies and pharmacological applications of calcitonin family molecules.
