Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Deconvolution01:20

Deconvolution

718
Deconvolution, also known as inverse filtering, is the process of extracting the impulse response from known input and output signals. This technique is vital in scenarios where the system's characteristics are unknown, and they must be inferred from the observable signals.
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...
718
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.9K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

15.3K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
15.3K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

2.0K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
2.0K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

1.0K
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
1.0K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

12.3K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
12.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analyzing the super-resolution characteristics of focused-spot illumination approaches.

Journal of biomedical optics·2020
Same author

Achieving superresolution with illumination-enhanced sparsity.

Optics express·2018
Same author

Electrowetting lenses for compensating phase and curvature distortion in arrayed laser systems.

Applied optics·2013
Same author

Noise removal in extended depth of field microscope images through nonlinear signal processing.

Applied optics·2013
Same author

Quantitative phase microscopy through differential interference imaging.

Journal of biomedical optics·2008
Same author

Quantitative structured-illumination phase microscopy.

Applied optics·2007

Related Experiment Video

Updated: Apr 12, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
07:12

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

697

Total variation regularized deconvolution for extended depth of field microscopy.

Ramzi N Zahreddine, Carol J Cogswell

    Applied Optics
    |May 14, 2015
    PubMed
    Summary

    Extended depth of field microscopy uses point spread function (PSF) engineering and digital deconvolution. This study presents a microscopy-specific model and total variation deconvolution for improved fluorescence imaging resolution.

    More Related Videos

    Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
    08:04

    Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

    Published on: March 12, 2017

    10.0K
    Sample Drift Correction Following 4D Confocal Time-lapse Imaging
    10:04

    Sample Drift Correction Following 4D Confocal Time-lapse Imaging

    Published on: April 12, 2014

    17.2K

    Related Experiment Videos

    Last Updated: Apr 12, 2026

    Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
    07:12

    Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

    Published on: January 6, 2026

    697
    Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
    08:04

    Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

    Published on: March 12, 2017

    10.0K
    Sample Drift Correction Following 4D Confocal Time-lapse Imaging
    10:04

    Sample Drift Correction Following 4D Confocal Time-lapse Imaging

    Published on: April 12, 2014

    17.2K

    Area of Science:

    • Optical Engineering
    • Microscopy
    • Image Processing

    Background:

    • Extending the depth of field in optical systems is crucial for improved 3D imaging.
    • Current methods often combine point spread function (PSF) engineering with post-processing.
    • Literature on applying these techniques to fluorescence microscopy is limited.

    Purpose of the Study:

    • To formalize a microscopy-specific imaging model for extended depth of field.
    • To experimentally demonstrate a total variation regularized deconvolution approach for fluorescence microscopy.
    • To compare the performance of this method against the Wiener filter.

    Main Methods:

    • Utilized a phase mask in the back aperture to create a focus-invariant PSF.
    • Developed a microscopy-specific imaging model.
    • Implemented and evaluated a total variation regularized deconvolution algorithm.

    Main Results:

    • Successfully demonstrated extended depth of field in fluorescence microscopy.
    • The total variation regularized deconvolution approach showed promising results in restoring transverse resolution.
    • Performance was compared to the conventional Wiener filter.

    Conclusions:

    • The combination of PSF engineering and advanced deconvolution techniques offers a viable method for extending depth of field in fluorescence microscopy.
    • Total variation regularization provides an effective approach for image restoration in this context.
    • Further research can build upon this model for enhanced microscopic imaging.