Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

12.3K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
12.3K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.9K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

1.0K
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
1.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

22.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
22.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interpretable Deep Learning for Single-Molecule Nanopore Fingerprinting Using Physics-Guided Preprocessing.

ACS sensors·2026
Same author

AI to Identify Strain-Sensitive Regions of the Optic Nerve Head Linked to Functional Loss in Glaucoma.

Investigative ophthalmology & visual science·2026
Same author

Near-field probing of the local density of optical states enhanced by bound states in the continuum in nonlocal metasurfaces.

Nature communications·2025
Same author

Integrated lithium niobate photonic computing circuit based on efficient and high-speed electro-optic conversion.

Nature communications·2025
Same author

All-optical scalable and programmable VCSEL-based Ising annealer with parallel feedback.

Optics express·2025
Same author

Sensitivity fields and parameter estimation from dielectric objects.

Journal of the Optical Society of America. A, Optics, image science, and vision·2025
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Apr 12, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.4K

Focusing and imaging in microsphere-based microscopy.

Thanh Xuan Hoang, Yubo Duan, Xudong Chen

    Optics Express
    |May 14, 2015
    PubMed
    Summary
    This summary is machine-generated.

    Microsphere microscopy achieves super-diffraction imaging by harnessing surface waves. Optimized beam polarization and geometry enable focusing light to sub-diffraction-limit spots for enhanced resolution.

    More Related Videos

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
    08:53

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

    Published on: August 15, 2014

    10.2K
    Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
    09:09

    Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

    Published on: December 17, 2015

    10.3K

    Related Experiment Videos

    Last Updated: Apr 12, 2026

    Conducting Multiple Imaging Modes with One Fluorescence Microscope
    08:32

    Conducting Multiple Imaging Modes with One Fluorescence Microscope

    Published on: October 28, 2018

    10.4K
    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
    08:53

    Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

    Published on: August 15, 2014

    10.2K
    Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
    09:09

    Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

    Published on: December 17, 2015

    10.3K

    Area of Science:

    • Optical Physics
    • Nanotechnology
    • Microscopy

    Background:

    • Microsphere-based microscopy is gaining attention for its ability to overcome the diffraction limit.
    • Existing systems show promise but require a deeper theoretical understanding of their optical performance.

    Purpose of the Study:

    • To develop a comprehensive theoretical model for microsphere-based microscopy systems.
    • To investigate the role of surface waves and beam characteristics in achieving super-resolution imaging.

    Main Methods:

    • Developed a complete theoretical model covering illumination, sample interaction, and imaging.
    • Simulated the focusing and imaging capabilities of microsphere systems.
    • Analyzed the impact of beam polarization and geometry on optical performance.

    Main Results:

    • Surface waves are crucial for focusing and imaging in microsphere systems.
    • Radially polarized convergent beams can achieve sub-diffraction-limit focusing.
    • Resolved two dipoles spaced 98 nm apart using a 402.292 nm wavelength light.
    • Beam geometry and polarization significantly influence focal spot size and shape.

    Conclusions:

    • The theoretical model provides a framework for understanding and optimizing microsphere microscopy.
    • Exploiting surface waves and tailored beam properties is key to achieving super-resolution.
    • This work paves the way for enhanced optical resolution in microscopy applications.