Lacinutrix undariae sp. nov., isolated from a brown algae reservoir

A Gram-stain-negative, aerobic, non-flagellated, non-gliding and ovoid or rod-shaped bacterium, designated strain W-BA8T, was isolated from a brown algae reservoir on the South Sea, South Korea, and subjected to a polyphasic taxonomic approach. Strain W-BA8T grew optimally at 25 °C, at pH 7.0-7.5 and in the presence of 1.0-2.0% (w/v) NaCl. Neighbour-joining and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain W-BA8T clustered with the type strains of species of the genus Lacinutrix. Strain W-BA8T exhibited 16S rRNA gene sequence similarity values of 94.9-96.5% to the type strains of Lacinutrix species and of less than 95.8% to the type strains of other recognized species. Strain W-BA8T contained MK-6 as the predominant menaquinone and iso-C15 : 0, iso-C15 : 1 G, iso-C15 : 0 3-OH and iso-C17 : 0 3-OH as major fatty acids. The polar lipid profile of strain W-BA8T contained phosphatidylethanolamine, two unidentified lipids and one unidentified glycolipid as major components. The DNA G+C content of strain W-BA8T was 35 mol%. Differential phenotypic properties, together with phylogenetic distinctiveness, revealed that strain W-BA8T is separated from other species of the genus Lacinutrix. On the basis of the data presented, strain W-BA8T is considered to represent a novel species of the genus Lacinutrix, for which the name Lacinutrix undariae sp. nov. is proposed. The type strain is W-BA8T ( = KCTC 42176T = CECT 8671T).