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Temperature dependent replication of porcine parvovirus isolates
C S Choi1, H S Joo, T W Molitor
1Department of Large Animal Clinical Sciences, University of Minnesota, St. Paul.
Archives of Virology
|January 1, 1989
Summary
Replication of porcine parvovirus isolates KBSH and Kresse showed temperature-dependent behavior in swine testes cells. KBSH replication was restricted at 39°C, while Kresse was restricted at lower temperatures.
Area of Science:
- Veterinary Virology
- Molecular Biology
Background:
- Porcine parvovirus (PPV) is a significant pathogen in swine.
- Understanding PPV isolate replication dynamics is crucial for disease control.
Purpose of the Study:
- To compare the replication of four PPV isolates (NADL-8, NADL-2, KBSH, Kresse) in swine testes cells.
- To investigate the effect of temperature on PPV isolate replication.
Main Methods:
- Culturing swine testes cells.
- Inoculating cells with four PPV isolates (NADL-8, NADL-2, KBSH, Kresse).
- Incubating cultures at 32°C, 37°C, and 39°C and assessing viral replication.
Main Results:
- Kresse isolate replication was restricted at 32°C and 37°C, but not at 39°C.
- KBSH isolate replication was restricted at 39°C, but not at 32°C or 37°C.
- NADL-8 and NADL-2 isolates showed no significant temperature-dependent replication restriction under tested conditions.
Conclusions:
- Replication of PPV isolates KBSH and Kresse is temperature-dependent.
- Different PPV isolates exhibit distinct temperature-dependent replication profiles.