Properties and Application of a Partially Purified Thermoalkali Stable Xylanase from Cellulosimicrobium sp. MTCC
Rajashri D Kamble1, Anandrao R Jadhav2
1Department of Biotechnology Engineering, Tatyasaheb Kore Institute of Engineering and Technology, Warananagar, Kolhapur, Maharashtra 416113, India.
Abstract:
The most promising application of xylanases (E.C. 3.2.1.8) is in the prebleaching of kraft pulp. The present paper reports bleaching effects of a thermoalkali stable xylanase from Cellulosimicrobium sp. MTCC 10645. The bacterium produced thermo-alkali stable xylanase in a basal medium supplemented with wheat bran (1% w/v), which was optimally active at pH 7.0 and 50°C. The xylanase was stable at temperature 50°C for 1 h and retained up to 86% of the activity. The xylanase was stable in a broad pH range of 6.0-11.0 for 1 h at 50°C. Metal ions Ca(+2), Hg(+2), and Pb(+2) were inhibitory for xylanase retaining 72.3%, 35.07% and 36.7% relative activity at 10 mM concentration, whereas Fe(+2), Cu(+2), Mn(+2), Na(+2), Co(+2), and Zn(+2) were inducers at concentrations of 5 mM and 10 mM. The enzyme exhibited greater binding affinity exclusively for xylans but not for avicel, CMC, cellobiose, starch, or p-nitrophenyl xylopyranoside. Parachloromercuric benzoate and iodoacetamide were found stimulatory, while potassium permanganate, cysteine, and cystine markedly reduced the activity. The xylanase dose of 2.0 U/g dry weight pulp of 10% consistency gave optimum bleach boosting of kraft pulp at pH 8.0 and temperature 50°C for 5 h reaction time.
