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Updated: Apr 12, 2026
An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells
Published on: December 16, 2019
A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues
Akon Higuchi1, Ching-Tang Wang2, Qing-Dong Ling3
11] Department of Chemical and Materials Engineering, National Central University, Jhong-li, Taoyuan, Taiwan [2] Department of Botany and Microbiology, King Saud University, Riyadh, Saudi Arabia [3] Department of Reproduction, National Research Institute for Child Health and Development, Tokyo, Japan [4] Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama, Japan.
A novel hybrid-membrane migration method achieves highly pure human adipose-derived stem cells (hADSCs). This technique enhances stem cell pluripotency and marker expression compared to traditional isolation.
Area of Science:
- Stem cell biology
- Tissue engineering
- Biomaterials science
Background:
- Human adipose-derived stem cells (hADSCs) display inherent heterogeneity.
- Cellular properties and differentiation potential vary based on isolation methods.
- Current purification techniques can result in inconsistent hADSC purity and characteristics.
Purpose of the Study:
- To develop an advanced purification method for hADSCs.
- To achieve high purity and enhanced pluripotency in isolated hADSCs.
- To compare the efficacy of the novel method against conventional isolation techniques.
Main Methods:
- A hybrid-membrane migration approach was employed.
- Fat tissue solution was permeated through porous membranes (8-25 μm pore size).
- Membranes were incubated in cell culture medium for 15-18 days to allow cell migration.
Main Results:
- The hybrid-membrane method yielded hADSCs with >98% purity for mesenchymal stem cell markers.
- Pluripotency gene expression (Oct4, Sox2, Klf4, Nanog) was significantly higher (nearly one order of magnitude).
- Isolated hADSCs demonstrated superior pluripotency compared to conventionally isolated cells.
Conclusions:
- The hybrid-membrane migration method is effective for high-purity hADSC isolation.
- This technique significantly enhances the pluripotency of hADSCs.
- The developed method offers a promising advancement for stem cell research and therapeutic applications.
