Related Experiment Video

Updated: Apr 12, 2026

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

10.6K

Identification of post-transcriptional regulatory networks during myeloblast-to-monocyte differentiation transition

Giulia Fontemaggi1, Teresa Bellissimo, Sara Donzelli

  • 1a Translational Oncogenomics Unit; "Regina Elena" National Cancer Institute ; Rome , Italy.

RNA Biology
|May 14, 2015
PubMed

Keywords:
AGO2, argonaute 2AMLAML, acute myeloid leukemiaECL methods, enhanced chemiluminescence methodsGAPDH, glyceraldehyde 3-phosphate dehydrogenaseGFP, green fluorescent proteinHPCs, haematopoietic progenitor cellsKPNA2, karyopherin α, 2NBT assay, nitroblue tetrazolium assayPLK1PLK1, polo-like kinase 1PMSF, phenylmethylsulfonyl fluorideRAB10, member RAS oncogene family 10RAB5C, member RAS oncogene family 5CRT-qPCR, quantitative reverse transcription polymerase chain reactionSF2A1, splicing factor 2A1TFs, transcription factorsVitD3, 1,25-dihydroxyvitamin D3miRNAs, microRNAsmicroRNAsmyeloid differentiationribosomal/polysomal fractions

More Related Videos

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation11:40

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

9.0K
Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis12:44

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

12.8K

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
8.0K
Master Transcription Regulators02:23

Master Transcription Regulators

2.9K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
4.3K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.6K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
27.4K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

4.3K