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Updated: Apr 12, 2026

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
Published on: June 27, 2014
A comprehensive study of cold protonated tyramine: UV photodissociation experiments and ab initio calculations
Michel Broquier1, Satchin Soorkia, Gilles Grégoire
1CNRS, Université Paris Sud, Institut des Sciences Moléculaires d'Orsay (ISMO) UMR 8214, 91405 Orsay Cedex, France. gilles.gregoire@u-psud.fr.
Abstract:
We present a comprehensive experimental study of protonated tyramine ions in a cold 3D quadrupole ion trap coupled to a time-of-flight mass spectrometer. Multiple UV photodissociation techniques have been developed, including single and double resonance spectroscopy along with time-resolved excited state lifetime measurements through a picosecond pump-probe scheme. An original UV-UV hole burning method is presented which can be used without modification of the quadrupole ion trap. The electronic spectrum of the cold protonated tyramine exhibits well-defined vibronic transitions, allowing the firm assignment of its two low-lying energy conformations by comparison with CC2 ab initio excited state calculations.
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