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Corticotropin-releasing factor-binding protein is a glycoprotein
1Department of Medicine, Tokyo Women's Medical College, Japan.
Biochemical and Biophysical Research Communications
|December 15, 1989
Summary
Human corticotropin-releasing factor-binding protein (hCRF-BP) is a glycoprotein. Its oligosaccharide chains are crucial for binding hCRF in plasma, as shown by enzymatic treatments.
Area of Science:
- Biochemistry
- Glycobiology
- Endocrinology
Background:
- Human corticotropin-releasing factor-binding protein (hCRF-BP) binds corticotropin-releasing factor (CRF) in plasma.
- The structural and functional characteristics of hCRF-BP are not fully understood.
Purpose of the Study:
- To investigate the structural nature of hCRF-BP.
- To determine the role of oligosaccharides in hCRF-BP's binding activity.
Main Methods:
- Adsorption of the CRF-BP-CRF complex to concanavalin-A-Sepharose.
- Enzymatic treatment of the complex with endoglycosidase H and glycopeptidase A.
- Analysis of molecular weight changes and binding affinity.
Main Results:
- The molecular weight of the CRF-BP-CRF complex decreased after enzymatic treatment.
- Treatment with endoglycosidase H reduced the binding affinity between CRF-BP and CRF.
- These findings suggest hCRF-BP is a glycoprotein with N-linked oligosaccharides.
Conclusions:
- hCRF-BP is a glycoprotein containing asparagine N-linked-type oligosaccharides.
- These oligosaccharide chains play a significant role in the binding of CRF-BP to CRF.