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Normalization of Affymetrix miRNA Microarrays for the Analysis of Cancer Samples
Di Wu1,2, Michael P Gantier3,4
1Department of Statistics, Harvard University, Cambridge, MA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 15, 2015
Abstract:
microRNA (miRNA) microarray normalization is a critical step for the identification of truly differentially expressed miRNAs. This is particularly important when dealing with cancer samples that have a global miRNA decrease. In this chapter, we provide a simple step-by-step procedure that can be used to normalize Affymetrix miRNA microarrays, relying on robust normal-exponential background correction with cyclic loess normalization.
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