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Single-use thermoplastic microfluidic burst valves enabling on-chip reagent storage.

Omid D Rahmanian¹, Don L DeVoe²

¹Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.

Microfluidics and Nanofluidics

|May 15, 2015

Summary

This study presents a novel method for creating single-use burst valves in thermoplastic microfluidic devices. These robust valves enable reliable on-chip reagent storage for point-of-care diagnostic assays.

Keywords:

Microfluidic valves Orogenic fabrication Point-of-care detection Reagent storage Thermoplastic fabrication

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Area of Science:

Materials Science
Microfluidics
Biomedical Engineering

Background:

Thermoplastic microfluidic devices are crucial for point-of-care diagnostics.
Fabricating reliable, integrated components like valves remains a challenge.
Existing methods may lack integration capabilities or robustness for reagent storage.

Purpose of the Study:

To develop a simple, reliable method for fabricating single-use normally closed burst valves in thermoplastic microfluidics.
To characterize the relationship between valve geometry and burst pressure.
To demonstrate the utility of these valves in an integrated microfluidic device for reagent storage and actuation.

Main Methods:

A novel fabrication process for burst valves compatible with existing thermoplastic microfluidic workflows.
Experimental investigation of valve performance, correlating geometric parameters with burst pressure.
Integration of multiple valves with varying burst pressures into a microfluidic device actuated by screw pumps.

Main Results:

Successful fabrication of single-use burst valves with predictable burst pressures based on geometry.
Demonstration of controlled actuation of multiple valves at distinct pressures.
On-chip hermetic storage and reconstitution of fluorescein salt within reagent chambers.

Conclusions:

The developed burst valve technology is simple, reliable, and integrates seamlessly into thermoplastic microfluidic fabrication.
The low permeability of cyclic olefin copolymer ensures robust, hermetic reagent storage.
This technology is highly suitable for developing integrated, disposable microfluidic assays for point-of-care applications.